Experimental Study of Antifungal Effect of Povidone Iodine Solution on Candida Albicans / 中华皮肤科杂志

The effect of povidone iodine solution(PIS)on the metabolic activity of Candida albi- cans and the effect of PIS treated C.altn'cans to cultured human keratinocytes were studied by using the Alamar blue fluorescence assay.It was found that PIS inhibited the metabolic activity of Candida albi- cans with a dose-dependent manner,and that the LD_(50)of PIS for Candida albicans is 0.075%.After treating for 30 min by 2% PIS/PBS(-),Candida albicans almost lost the adherence and invasion to the cultured human keratinocytes,the wall of yeast cells became uneven and shrunken,the intracellular structures became obviously indistinct,and the yeasts could not develop to hypha form.Candida albi- cans,treated with 2% PIS in PBS(-)for 30 min or with 10% PIS Sabouraud's liquid medium for 33 hr,did not form colony when it was inoculated to Sabouraud's dextrose agar.This result suggests that the fungicidal effective of PIS is influenced by its solvent.

Construction of an Eukaryotic Expression Vector pcDNA3-Glutathione-S-Transferase A1 Gene From Human Liver cDNA Library and Partial Sequence Determination / 环境与健康杂志

Objective To construct an eukaryotic expression vector carrying human glutathione-S-transferase(GST)A1gene and to provide study materials for toxicology and pharmacogenetic.Methods The GST A1cDNA was amplified and separated from human liver total RNAs by RT-PCR approach and recombined with eukaryotic expression vector pcDNA3.The recombined plasmid pcDNA3-hGSTA1was verified using PCR,restriction analysis and sequencing determination.Results Human GST A1gene was recombined correctly with pcDNA3,compared with Genbank,in code152T→C,amino acid Met→Thr.Conclusion The eukaryotic expression vector pcDNA3-hGSTA1was constructed for research on toxicology and pharmacogenetics.